Training School CLEM, STEM, EDS

October 4th - 6th, 2021, virtual event

This meeting will be hosted by Roland Fleck (Centre for Ultrastructural Imaging, King’s College London) and Pedro Machado (Centre for Ultrastructural Imaging, King’s College London) and held virtually.

This Training School will accept a maximum number of 20 participants to enhance interaction.

PROGRAM

Day 1

Welcome session, selected student flash talks, biological specimen preparation practicals, round table discussion


AM - Pre-recorded Talks and demos*

High Pressure Freezing
Freeze Subsbitution
Sectioning
Freeze drying


PM - Live sessions

13h00 Welcome session

13h15 Students flash talks (part 1)

13h45 - 14h break

14h00 - Students flash talks (part 2)

14h45 - 15h break

15h00 - 16h30 Panel discussion and Q&A Specimen preparation
16h30 - 17h Networking session
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Day 2

Multimodal Imaging (LM, STEM) talks and practicals, round table discussions

AM - Pre-recorded Talks and demos*
JEOL F200 STEM imaging

STEM tomography

Light microscopy
Data correlation with ec-CLEM

Processing of STEM tomo data with IMOD



PM - Live sessions

13h00 - 14h00 Panel discussion and Q&A STEM imaging

14h00 - 14h15 break

14h15 - 14h45 ec-CLEM Live Demo

14h45 - 15h break

15h- 16h30 Panel discussion and Q&A Correlation and tomography
16h30 - 16h45 break

16h45 - 17h30 Troubleshooting/ open discussions
———————————————


Day 3

Multimodal Imaging (STEM, EDS)

AM - Pre-recorded Talks and demos*
Dragonfly segmentation and volume rendering
AZtec: data acquisition and analysis

PM - Live sessions

13h00 - 14h00 Panel discussion and Q&A AZtec: data acquisition and analysis

14h00 - 14h15 break

14h15 - 15h15 Panel discussion and Q&A Segmentation and volume rendering

15h15 - 15h30 break

15h30- 16h15 Troubleshooting/ open discussions
16h15 - 17h00 Wrap-up session



*all pre-recorded material will be made available on Day 1 and can be viewed by participants when convenient.

Organisers:

Roland Fleck - Centre for Ultrastructural Imaging, King’s College London

Pedro Machado - Centre for Ultrastructural Imaging, King’s College London

Key Note Speakers:

Roland Fleck

Angus Kirkland (University of Oxford)

Alex Sheader (University of Oxford)

Perrine Paul-Gilloteaux (SFR-Santé, INSERM, CNRS, UNIV Nantes, CHU Nantes)

Speakers:

Alex Sheader (University of Oxford)

Pedro Machado

Bartosz Marzec (JEOL)

Louise Hughes and Sam Marks (Oxford instruments)

Trainers:

Roland Fleck

Pedro Machado

Matt Russell (Centre for Ultrastructural Imaging, King’s College London)

Alejandra Carbajal (Centre for Ultrastructural Imaging, King’s College London)

Leanne Allison (Centre for Ultrastructural Imaging, King’s College London)

Bartosz Marzec

Suwimon Boonrungsiman (Centre for Ultrastructural Imaging, King’s College London)

Perrine Paul-Gilloteaux

Louise Hughes

Sam Marks

If you have any further questions, please contact us here.